L-type Ca2+ channels and SK channels in mouse embryonic stem cells and their contribution to cell proliferation
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Araujo, Raquel; Martín, Franz; Soria, Bernat; Vegara Meseguer, Josefina María; Pérez Sánchez, HoracioFecha
2015-01-23Disciplina/s
MedicinaMateria/s
Embryonic stem cellsIon channels
L-type Ca2+ channels
SK channels
Proliferation
Resumen
Mouse embryonic stem cells (mESCs) are capable of both self-renewal and
multilineage differentiation; thus, they can be expanded in vivo or in vitro and
differentiated to produce different cell types. Despite their biological and medical
interest, many physiological properties of undifferentiated mESCs, such as ion channel
function, are not fully understood. Ion channels are thought to be involved in cell
proliferation and differentiation. The aim of this study was to characterize functional ion
channels in cultured undifferentiated mESCs and their role in cell proliferation. L-type
voltage-activated Ca2+ channels sensitive to nifedipine and small conductance Ca2+-
activated K+ (SK) channels sensitive to apamin were identified. Ca2+-activated K+
currents were blocked by millimolar concentrations of tetraethylammonium (TEA). The
effects of Ca2+ channel and Ca2+-activated K+ channel blockers on the proliferation of
undifferentiated mESCs were investigated by bromodeoxyuridine ...