Lactate Biosensing for Reliable On-Body Sweat Analysis
Fecha
2021Disciplina/s
FarmaciaMateria/s
Lactate biosensorDiffusion limiting membranes
Real-time monitoring
Sweat analysis
Wearable sensors
Resumen
Wearable lactate sensors for sweat analysis are
highly appealing for both the sports and healthcare fields.
Electrochemical biosensing is the approach most widely used for
lactate determination, and this technology generally demonstrates
a linear range of response far below the expected lactate levels in
sweat together with a high influence of pH and temperature. In this
work, we present a novel analytical strategy based on the restriction
of the lactate flux that reaches the enzyme lactate oxidase, which is
immobilized in the biosensor core. This is accomplished by means
of an outer plasticized polymeric layer containing the quaternary
salt tetradodecylammonium tetrakis(4-chlorophenyl) borate (traditionally
known as ETH500). Also, this layer prevents the enzyme
from being in direct contact with the sample, and hence, any
influence with the pH and temperature is dramatically reduced. An expanded limit of detection in the millimolar range (from 1 to 50
mM) is demonstrated ...