Generation of RRMS and PPMS specific iPSCs as a platform for modeling Multiple Sclerosis
Author/s
Mutukula, Naresh; Man, Zhiqiu; Takahashi, Yuta; Iniesta Martinez, Francisca; Morales, Mariana; [et al.]Date
2021Discipline/s
MedicinaAbstract
The advent of cellular reprogramming technology converting somatic cells into induced pluripotent stem cells (iPSCs) has revolutionized our understandings of neurodegenerative diseases that are otherwise hard to access and model. Multiple Sclerosis (MS) is a chronic demyelinating, inflammatory disease of central nervous
system eventually causing neuronal death and accompanied disabilities. Here, we report the generation of several relapsing-remitting MS (RRMS) and primary progressive MS (PPMS) iPSC lines from MS patients along with their age matched healthy controls from peripheral blood mononuclear cells (PBMC). These patient
specific iPSC lines displayed characteristic embryonic stem cell (ESC) morphology and exhibited pluripotency marker expression. Moreover, these MS iPSC lines were successfully differentiated into neural progenitor cells (NPC) after subjecting to neural induction. Furthermore, we identified the elevated expression of cellular
senescence hallmarks in RRMS and PP...