dc.contributor.author | De Pablos Torró, Luis Miguel | |
dc.contributor.author | Gónzalez Gónzalez, Gloria | |
dc.contributor.author | Solano Parada, Jennifer | |
dc.contributor.author | Seco, Víctor | |
dc.contributor.author | Díaz Lozano, Isabel María | |
dc.contributor.author | Gómez Samblás, María Mercedes | |
dc.contributor.author | Cruz Bustos, Teresa | |
dc.contributor.author | Osuna, Antonio | |
dc.date.accessioned | 2024-02-08T15:15:49Z | |
dc.date.available | 2024-02-08T15:15:49Z | |
dc.date.issued | 2011 | |
dc.identifier.citation | De Pablos LM, González GG, Solano Parada J, Seco Hidalgo V, Díaz Lozano IM, Gómez Samblás MM, Cruz Bustos T, Osuna A. Differential expression and characterization of a member of the mucin-associated surface protein family secreted by Trypanosoma cruzi. Infect Immun. 2011 Oct;79(10):3993-4001. doi: 10.1128/IAI.05329-11. Epub 2011 Jul 25. PMID: 21788387; PMCID: PMC3187265. | es |
dc.identifier.uri | http://hdl.handle.net/10952/7297 | |
dc.description.abstract | We describe the characterization, purification, expression, and location of a 52-kDa protein secreted during
interaction between the metacyclic form of Trypanosoma cruzi and its target host cell. The protein, which we
have named MASP52, belongs to the family of mucin-associated surface proteins (MASPs). The highest levels
of expression of both the protein and mRNA occur during the metacyclic and bloodstream trypomastigote
stages, the forms that infect the vertebrate host cells. The protein is located in the plasma membrane and in
the flagellar pockets of the epimastigote, metacyclic, and trypomastigote forms and is secreted into the medium
at the point of contact between the parasite and the cell membrane, as well as into the host-cell cytosol during
the amastigote stage. IgG antibodies specific against a synthetic peptide corresponding to the catalytic zone of
MASP52 significantly reduce the parasite’s capacity to infect the host cells. Furthermore, when the protein is
adsorbed onto inert particles of bentonite and incubated with a nonphagocytic cell culture, the particles are
able to induce endocytosis in the cells, which seems to demonstrate that MASP52 plays a role in a process
whereby the trypomastigote forms of the parasite invade the host cell. | es |
dc.language.iso | en | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Differential expression and characterization of a member of the mucin-associated surface protein family secreted by Trypanosoma cruzi | es |
dc.type | article | es |
dc.rights.accessRights | openAccess | es |
dc.journal.title | Infection and immunity | es |
dc.volume.number | 79 | es |
dc.description.discipline | Medicina | es |
dc.identifier.doi | 10.1128/IAI.05329-11 | es |