Screening of Antibiotics Biodegradability from Wastewater
Date
2023-06-26Discipline/s
Ciencias AmbientalesSubject/s
WastewaterAntibiotic
Antiseptics
Biodegradability
ATR-FTIR spectroscopy
LCMS/MS metabolites
Abstract
Objective: One of the sources of environment antibiotics contamination is wastewater treatment plants (WWTPs), there by constituting a global public health risk. This present study aimed to investigate the biodegradability of antibiotics and antiseptics and highlights the biodegradation of Ciprofloxacin as a sole carbon source by a bacterium isolated from the sludge "El Kouwaer," WWTPlocated in Mascara. Materials and Methods: In the present study, biodegradability of some antibiotics and antiseptic were tested at 50 mg/l concentration through active sludge microorganisms by Manometric Respirometry Method (OECD 301F). Further analysis of 16S rRNA gene sequencing used to identify MK4 strain isolated from the sludge. Furthermore, ATR-FTIR spectroscopy was conducted in order to identify its biodegradation in the presence of different carbon sources and LCMS/MS spectrometry were used to identify the metabolite degradation. Results: Our Results revealed that four antibiotics tested were read...